CRISPR Screen Dataset
Liang J (2020) - 2-PMID31551363
Title: Genome-Wide CRISPR-Cas9 Screen Reveals Selective Vulnerability of -Mutant Cancers to WEE1 Inhibition.
Screen Details:
- Screen Rationale : ATRX KO synthetic lethal genes
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : PLC/PRF/5 [BTO:0003492, CELLOSAURUS:CVCL_0485, DEPMAP:ACH-001318]
- Phenotype : Cell Proliferation
- Condition : Mutation: ATRX (ETG546) KO
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 58 | Full Dataset Size: 19,062
- Experimental Setup : Timecourse | Duration: 14 Doublings | MOI: ~ 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes determined to be lethal in a WT background have been removed from the hits in order to isolate genes that are synthetic lethal with ATRX
Score Distribution
< 1.0306
> -1.476