CRISPR Screen Dataset
Banik SM (2020) - 1-PMID32728216
Title: Lysosome-targeting chimaeras for degradation of extracellular proteins.
Screen Details:
- Screen Rationale : Genes involved in lysosome delivery by LYTACS
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRi-v2 (inhibition) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 52 | Full Dataset Size: 18,657
- Experimental Setup : Timecourse | Duration: 1 Hours | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- genes for which knockdown ablates the delivery of NA-647 by biotinylated LYTACs
Score Distribution
< 12.09312647
> 0.0000434