CRISPR Screen Dataset

Jost M (2017) - 7-PMID28985505

H. sapiens

Title: Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.

Screen Rationale : Gene inhibition affects response to chemical
Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
Phenotype : Response To Chemicals
Condition : blebbistatin (50.0 µM) [ChEBI]
Library : CRISPRi Targeted Library (Weissman, 2017) | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 316 | Full Dataset Size: 1,028
Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: < 1.0
Significance : Authors have indicated that results with Score.1 (Rho (Log2e Treated vs. Untreated)) > 0.15 OR Score.1 (Rho (Log2e Treated vs. Untreated)) < -0.15 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

inhibition of hit genes results in decreased sensitivity to blebbistatin (positive rho score) or increased sensitivity toblebbistatin (negative rho score)

< 0.789325042

> -0.290608786