CRISPR Screen Dataset

Heaton BE (2017) - 1-PMID28813663

H. sapiens

Title: A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor.

Screen Rationale : Gene activation results in increased resistance to virus
Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
Phenotype : Response To Virus
Condition : Virus: Influenza A (A/Puerto Rico/8/1934(H1N1) (5 MOI) [NCBI Taxid 211044]
Library : CRISPRa (Heaton, 2017) | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
Analysis Method : Ss=log10(10,000/Ranked position based on abundance) | Number of Hits: 25 | Full Dataset Size: 18,866
Experimental Setup : Virus Exposure | Duration: 5 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Log10) > 4.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

The authors used a ZsGreen reporter to identify cells in which activation of a gene results in the cell being refractory to IAV infection
These cells were then sequenced to identify enriched sgRNAs
Activation of the gene=protective effect, gene normally plays a protective role against infection

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