CRISPR Screen Dataset
Diep J (2019) - 1-PMID31527793
Title: Enterovirus pathogenesis requires the host methyltransferase SETD3.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Cell Line : H1-HeLa+CHDR3 [CELLOSAURUS:CVCL_C8ZH]
- Phenotype : Response To Virus
- Condition : Virus: Human rhinovirus C15 (RV-C15) (1 MOI) [NCBI Taxid 1418033]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,013 | Full Dataset Size: 21,810
- Experimental Setup : Virus Exposure | Duration: 6 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes has a protective effect in the presence of rhinovirus C on H1-HeLa+CHDR3 which are susceptible to the virus
Score Distribution
< 1
> 2.55e-16