CRISPR Screen Dataset

Hyrina A (2019) - 2-PMID31801065

H. sapiens

Title: A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production.

Screen Rationale : Anti-HBsAg (hepatitis B virus surface antigen) expression genes
Cell Type : Hepatitis B Virus Genome Integrated Cell Line
Cell Line : HepG2-NTCP1 HBV Cas9 [CELLOSAURUS:CVCL_C9BB]
Phenotype : Response To Virus
Library : CRISPRn (Helliwell, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : RSA | Number of Hits: 38 | Full Dataset Size: 19,049
Experimental Setup : Timecourse | Duration: 10 Days | MOI: = 0.05
Significance : Authors have indicated that results with Score.1 (Log2) > 1.0 AND Score.2 (RSA) < -4.94 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

authors stained Hep-G2(HBV) cells with an HBsAg antibody and sorted into high HBsAg and low HBsAg populations
enrichment of sgRNA-targeted genes was compared between populations expressing low and high levels of HBsAg. This analysis identified 38 genes that appeared to inhibit HBsAg expression ("anti-HBsAg")

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