CRISPR Screen Dataset
Alimov I (2019) - 1-PMID30647128
Title: Bile acid analogues are activators of pyrin inflammasome.
Screen Details:
- Screen Rationale : Inflammasome activation genes
- Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
- Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
- Phenotype : Pyroptosis
- Condition : BAA473 (11-oxo-12S-hydroxylithocholic acid methyl ester) (75 µM) [ChemSpider]
- Library : CRISPRn (Hoffman and Nyfeler, 2016) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 141 | Full Dataset Size: 19,049
- Experimental Setup : Drug Exposure | Duration: 9 Days
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Inflammasome induced pyroptosis (inflammasome-associated cell death) was used as a read-out
Score Distribution
< -0.000885412
> -18.24125232