CRISPR Screen Dataset
Haney MS (2018) - 2-PMID30397336
Title: Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens.
Screen Details:
- Screen Rationale : Phagocytosis genes
- Cell Type : Lymphoma Cell Line [BTO:0000104]
- Cell Line : U-937 [BTO:0001412, CELLOSAURUS:CVCL_0007]
- Phenotype : Phagocytosis
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 84 | Full Dataset Size: 20,502
- Experimental Setup : Timecourse | Duration: 5 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.1 (CasTLE Score) > 22.6 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors exposed cells to 1.3 um diameter superparamagnetic beads then used magnetic selection and sequencing to identify cells where particle uptake (phagocytosis) was affected.
- gRNAs with positive combo effects are enriched in the magnetically bound fraction and represent genes that are necessary for efficient phagocytosis, gRNAs with a negative CASTLE effect are are depleted in the magnetically bound fraction and represent genes that inhibit phagocytosis
Score Distribution
< 320
> 0