CRISPR Screen Dataset
Haney MS (2018) - 1-PMID30397336
Title: Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens.
Screen Details:
- Screen Rationale : Phagocytosis genes
- Cell Type : Lymphoma Cell Line [BTO:0000104]
- Cell Line : U-937 [BTO:0001412, CELLOSAURUS:CVCL_0007]
- Phenotype : Phagocytosis
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 101 | Full Dataset Size: 20,500
- Experimental Setup : Timecourse | Duration: 5 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.1 (CasTLE Score) > 21.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors exposed cells to 4.3 um diameter superparamagnetic beads then used magnetic selection and sequencing to identify cells where particle uptake (phagocytosis) was affected.
- gRNAs with positive combo effects are enriched in the magnetically bound fraction and represent genes that are necessary for efficient phagocytosis. Genes with negative combo effects are depleted in the magnetically bound fraction and represent genes that inhibit phagocytosis
Score Distribution
< 128
> 0