CRISPR Screen Dataset
Gulbranson DR (2019) - 1-PMID31353312
Title: AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
Screen Details:
- Screen Rationale : Endocytosis genes
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,021 | Full Dataset Size: 18,737
- Experimental Setup : Timecourse | Duration: 2 Hours
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.03 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- the authors use HA-GLUT4-GFP as a reporter for clathrin-mediated endocytosis
Score Distribution
< 0.99996
> 2.66e-16