CRISPR Screen Dataset
Liu X (2019) - 2-PMID31219803
Title: A large-scale CRISPR screen and identification of essential genes in cellular senescence bypass.
Screen Details:
- Screen Rationale : Senescence genes
- Cell Type : Fibroblast Cell Line [BTO:0000453]
- Cell Line : BJ [BTO:0003807, CELLOSAURUS:CVCL_3653]
- Phenotype : Senescence
- Condition : Bleomycin (40 µg/mL) [ChEBI]
- Library : CRISPRn Senescence Targeted Library (Wang, 2019) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MA-plot | Number of Hits: 30 | Full Dataset Size: 1,416
- Experimental Setup : Drug Exposure | Duration: 27 Days | MOI: = 0.22
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- The author states that 30 genes found to be significantly enriched (p<0.05) in two replicates represent candidate senescence genes
Score Distribution
< 6.828192571
> -1.669542991