CRISPR Screen Dataset

Huang H (2019) - 1-PMID31165872

H. sapiens

Title: A CRISPR/Cas9 screen identifies the histone demethylase MINA53 as a novel HIV-1 latency-promoting gene (LPG).

Screen Rationale : HIV latency promoting genes
Cell Type : HIV-1 Latency Cell Line
Cell Line : J-Lat Tat-GFP Clone A2 [CELLOSAURUS:CVCL_1G43]
Phenotype : Protein/peptide Accumulation [APO:0000149]
Library : Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : HiTSelect | Number of Hits: 446 | Full Dataset Size: 3,733
Experimental Setup : Timecourse | Duration: 7 Days | MOI: ~ 0.4
Significance : Authors have indicated that ALL results are to be considered significant.

authors screened J-Lat A2 cells harboring a latently infected 'LTR-Tat-IRES-GFP' HIV-1 minigenome using the nuclear subpool library. Hits represent genes whose loss led to activation of the Tat-GFP reporter, signifying reactivation of the latent virus

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