CRISPR Screen Dataset
Liu J (2019) - 2-PMID31316073
Title: Pooled library screening with multiplexed Cpf1 library.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,536 | Full Dataset Size: 18,663
- Experimental Setup : Timecourse | Duration: 4 Weeks | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a Scaled Bayesian Factor (BF) greater than 0 were identified as fitness genes (hits) for this cell line in this CRISPR screen. A cutoff threshold for gene hit identification was set to a false discovery rate (FDR) of 0.01. An SpCas9-based human mono-cistronic library was used.
Score Distribution
< 34.7860666666667
> -27.2936