CRISPR Screen Dataset
Liu J (2019) - 1-PMID31316073
Title: Pooled library screening with multiplexed Cpf1 library.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Library : Mini-human [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: AsCpf1 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,594 | Full Dataset Size: 16,393
- Experimental Setup : Timecourse | Duration: 4 Weeks | MOI: < 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a Scaled Bayesian Factor (BF) greater than 0 were identified as fitness genes (hits) for this cell line in this CRISPR screen. A cutoff threshold for gene hit identification was set to a false discovery rate (FDR) of 0.01. An AsCpf1-based human multiplexed library was used with the optimized AsCpf1-3xMYC variant.
Score Distribution
< 35.6208
> -27.2936