CRISPR Screen Dataset
Chang SJ (2019) - 2-PMID30951565
Title: Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Response To Toxin
- Condition : Typhoid Toxin (40.0 pM)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 27 | Full Dataset Size: 19,030
- Experimental Setup : Toxin Exposure | Duration: 1 Hours
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.15 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- these results are from a screen using the Geckov2 sub-pool B library to screen HEK293T cells for genes whose loss confers resistance to typhoid toxin
Score Distribution
< 0.99998
> 0.000000000131