CRISPR Screen Dataset
Flint M (2019) - 1-PMID30655525
Title: A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5.1 [BTO:0005463, CELLOSAURUS:CVCL_E049]
- Phenotype : Response To Virus
- Condition : Virus: Ebola virus (Mayinga strain) (0.3 MOI) [NCBI Taxid 128952]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 538 | Full Dataset Size: 16,985
- Experimental Setup : Virus Exposure | Duration: 3 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- The authors exposed cells to the Ebola virus (Mayinga strain) and looked for cells whose sgRNA were enriched in the exposed cell pool, indicating loss of knock-out of the gene target protects cells from infection and subsequent death
Score Distribution
< 1
> 0.00000000000000137